Figure 3.

(A) FTIR spectra (left) and corresponding second derivatives (right) of oxidized (black lines) and reduced amyloid fibrils (red lines) were recorded using the attenuated total reflection in a D₂O buffer at pD 6.0. The FTIR spectra are normalized with respect to the peak intensity at 1621 cm⁻¹. Three spectra obtained from different preparations of amyloid fibrils are shown. (B) PK-resistant fragments of PrP monomers (left) and amyloid fibrils (right) were analyzed by tricine-SDS-PAGE and CBB staining. Here, protein monomers or amyloid fibrils (12.5 μM in the monomer equivalent units) were treated with 0 (lane 1), 0.0125 (lane 2), 0.125 (lane 3), or 1.25 μM PK (lane 4) at 37°C for 1 h at pH 7.4 in the presence of 1 M GuHCl. To see this figure in color, go online.