Figure 1.

Different distributions of GFP-RyR2 clusters in the periphery and interior of live isolated ventricular myocytes and intact hearts. Representative confocal images are from the z-stack of (A) live ventricular myocytes (n = 10) and (B) intact hearts (n = 14 scans from three hearts) isolated from mice expressing a GFP-tagged RyR2. Imaging focusing on the interior showed highly ordered arrays of GFP-RyR2 clusters (green puncta) with clear single transverse rows in both live isolated cells and tissue (Aa and Ba). In contrast, optical confocal z-planes near the periphery showed irregular distribution of RyR2 clusters within the same cell/tissue (Ab and Bb). Enlarged images of selected areas are shown on the right. To see this figure in color, go online.