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. 2018 Feb 3;114(2):343–354. doi: 10.1016/j.bpj.2017.11.026

Figure 3.

Figure 3

Time-lapse TIRF imaging of dynamics of GFP-RyR2 clusters in the periphery and interior of live ventricular myocytes. Representative images are given from time-lapse series of interior and peripheral GFP-RyR2 clusters, recorded using a TIRF microscope. Arrays of ordered GFP-RyR2 clusters were found in the interior. All GFP-RyR2 fluorescence signals (clusters 1–8) in the interior remained stationary (A, a and b). TrackMate advanced cluster dynamic analysis showed only minimal x-, y-signal movements (A, c and d, blue tracks). In contrast, distribution of signals appeared disordered in the periphery (B, a–e). The majority of peripheral GFP-RyR2 signals remained at same positions (B, a–e; f and g, blue tracks). However, multiple clusters displayed dynamic movements: 1) Translocation along the z axis (B, a–c, zoom: pink dashed circle); 2) GFP-RyR2 cluster fusion (B, d and e, zoom: turquoise dashed circle); and 3) unit separation (B, b–e, zoom: purple dashed circle). Additionally, TrackMate analysis showed major xy movement (B, f, red track) (n = 5 cells). To see this figure in color, go online.