Figure 4.

Movements of GFP-RyR2 clusters in the periphery of live ventricular myocytes are affected by RyR2 modulators. Representative images from time-lapse TIRF series of peripheral GFP-RyR2 clusters were recorded in the presence of (A) 2 mM extracellular Ca²⁺, (B) 2 mM extracellular Ca²⁺ plus 2 mM tetracaine, (C) 0.5 mM extracellular Ca²⁺, and (D) 2 mM extracellular Ca²⁺ plus 1 mM caffeine. Representative GFP-RyR2 cluster separation/fusion is highlighted (yellow dashed circles). (E) Given here is the fraction of cells that showed GFP-RyR2 cluster movement (n = 8–9 cells). To see this figure in color, go online.