Table 2.
Rotational Hydrodynamic Radii for Folded Proteins
| Protein (PDB ID) | M (Da) | RR,expa (Å) | RR,calc (Å) | % Signed Error | a/bb | Reference | |
|---|---|---|---|---|---|---|---|
| BPTI (5PTI) | 6518 | 15.3 | 15.9 | +3.73 | 1.66 | (65) | |
| Calbindin (1IG5) | 8502 | 17.0 | 16.6 | −2.29 | 1.10 | (98) | |
| Ubiquitin (1UBQ) | 8566 | 16.8 | 17.3 | +2.68 | 1.49 | (7) | |
| Cytochrome c (351C) | 8696 | 17.1 | 16.8 | −2.22 | 1.10 | (99) | |
| Plastocyanin (1PCS) | 10,322 | 18.0 | 17.6 | −2.06 | 1.19 | (100) | |
| Binase (1GOU) | 12,214 | 19.7 | 19.3 | −2.03 | 1.27 | (101) | |
| Ribonuclease A (1AQP) | 13,692 | 20.0 | 20.4 | +1.65 | 1.34 | (65) | |
| Azurin (1E5Y) | 13,948 | 19.7 | 19.7 | +0.25 | 1.31 | (102) | |
| Lysozyme (1AKI) | 14,315 | 19.2 | 20.1 | +5.06 | 1.51 | (65) | |
| Spo0F (2FSP) | 14,230 | 20.1 | 20.2 | +0.25 | 1.24 | (103) | |
| DHFR (1DYI) | 18,003 | 22.6 | 22.0 | −2.87 | 1.20 | (104) | |
| β-lactoglobulin (2AKQ) | 17,104 | 22.1 | 21.3 | −3.48 | 1.21 | (105) | |
| Apo-AK (4AKE) | 23,589 | 26.1 | 26.2 | +0.31 | 1.43 | (106) | |
| Holo-AK (1AKE) | 24,616 | 23.7 | 23.9 | +0.93 | 1.14 | (106) | |
| mean % signed error | −0.08 | ||||||
DHFR, dihydrofolate reductase.
a
Experimental hydrodynamic coefficients (τc, DR) as reported in the references were converted to RR for comparison.
b
Axial ratio of a prolate ellipsoid with volume equal to the initial convex hull volume.