Figure 4.

Single-molecule diffusion properties measured with single-molecule spectral imaging and analyzed with vbSPT (5). The diffusion state graphs obtained from single-color (top) and three-color (bottom) experiments are shown for WGA-CF633 (left), CellMask DR (middle), and HT-CF680R (right) as connected circles. Size of a circle indicates probabilities of the molecules residing in that specific state. Diffusion constants of state i are labeled as D_i (units: μm²/s), probability of molecules in state i is P_i, and the probability of transitioning from state i to state j within one frame is labeled as p_ij.