Figure 1.

Disaggregation of TMR-Aβ42 amyloids monitored by FCS in PBS (A–C) and in 4 M GdnCl (D–F). (A and D) FCS autocorrelation data, G(τ) (open circles) and the fits using Eq. 1 assuming single diffusing species (solid lines). Autocorrelation data were recorded every 30 s. However, traces recorded every 2 min are shown here for clarity of presentation. (B and E) Photon count rates (squares) and concentrations (circles) obtained from analysis of the autocorrelation data. (C and F) Circles represent hydrodynamic radii (R_h) obtained from analysis of the autocorrelation data, and the solid lines represent the mean of all the measured values of R_h. Total concentration of amyloids used here is 60 nM (monomer equivalent). Clearly, R_h of solubilized TMR-Aβ corresponds to the size of the monomeric peptide (see Fig. S2). To see this figure in color, go online.