Figure 5.
nsFCS measurement of Avi-Aβ40 and Avi-Aβ42. (A) Fluorescence bursts with FRET efficiencies between 0.4 and 0.8 (shaded in orange) were analyzed. (B) Global fitting of three correlation data (autocorrelation of the donor and acceptor and cross correlation) results in the conformational fluctuation timescale (τCD) and σc2 values of 30 (±2) ns and 0.059 (±0.003) for Aβ40 and 37 (±2) ns and 0.057 (±0.002) for Aβ42. σc2 values are consistent with the value 0.07 obtained from the 2D FRET efficiency-lifetime analysis. To see this figure in color, go online.