Table 2.

Rate Constants Obtained for Collagen Bead Detachment

Experiment	keff (min−1)	kns (min−1)	kTr (min−1)
− trypsin, F = 0	–	0.010a ± 0.013	–
+ trypsin, F = 0	0.020 ± 0.001	0.010b ± 0.013	0.009 ± 0.013
− trypsin, F = 9 pN	–	0.135 ± 0.003	–
+ trypsin, F = 9 pN	0.357 ± 0.018	0.135b ± 0.003	0.222 ± 0.018

Rate constants were determined by weighting <f(t)> by the mean ± SE.

^aDetermined by weighting f(t) by counting error (Table S2), as the mean ± SE-based k_ns = 0.007 min⁻¹ carried a very high uncertainty.

^bValues from the no-enzyme fits were used to determine the trypsin-dependent rate constants, using Eqs. 1 and (2a), (2b).