Figure S4(A) Strategy and subsequent experiments used to analyze the effect of Cdkn2a on browning. (B) Bodipy staining of differentiated ingWAT, eWAT and BAT adipocytes from Cdkn2a+/+ and Cdkn2a−/− mice. (C) mRNA levels for adipogenic and thermogenic genes in BAT primary adipocytes isolated from Cdkn2a+/+ and Cdkn2a−/− mice after 8 days induction of differentiation (n = 3). (D, E) Western blot assays (D) and their quantification (E) showing PKA substrates in Cdkn2a+/+ and Cdkn2a−/− ingWAT tissue homogenates. Tubulin was used as loading controls. All values are expressed as means ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001. Related to Figure 4.