Figure 5.

Mice deficient of LepR in brain barriers (LepR^beKO) are more sensitive to food reward. (A) Schematic illustration of the conditioned place preference setup. The chamber in which mice spent less time at baseline was paired with sucrose pellets. (B) After a conditioning phase of 8 days, mice spent more time in the chamber conditioned to sucrose. Conditioning was more effective in LepR^beKO mice than in LepR^Fl controls. Values represent means ± SEM. Repeated two-way ANOVA, for interaction F_{(1, 15)} = 6.182, p = 0.0252; for conditioning F_{(1, 15)} = 41.05. *p < 0.05, ***p < 0.0001 (Bonferroni posttest n = 7–10 mice/group). (C) Schematic illustration of the conditioned bottle choice test, wherein sucrose solution was offered in the bottle less preferred by each mouse at baseline. On alternating days, water was provided in the other bottle. (D) After 8 days of conditioning, LepR^beKO mice showed a higher preference for the bottle containing sucrose during the conditioning than LepR^Fl control animals. Repeated two-way ANOVA, for interaction F_{(1, 11)} = 6.263, p = 0.0294, for conditioning F_{(1, 11)} = 53.78, p < 0.0001. *p < 0.05, ***p < 0.0001 (Bonferroni posttest n = 6–7 mice/group).