Supplementary Figure S2.

Insulin secretion in EndoC-βH1 monolayer cells in response to incretins, incretin inhibitors and neuropeptides. A) Insulin secretion in response to 100 nM Ex4, 100 nM Liraglutide, and 100 nM GIP in addition to 11.2 mM glucose (high glucose), n = 4–8; B) Effect of the GLP1R antagonist Exendin9-39 used in concentrations at 0.1, 1, 10, 100 nM in addition to 15 mM glucose and 10 nM Ex4, n = 3. C) Inhibition of the incretin effect by shRNA mediated knockdown of the GLP1R in EndoC-βH1 cells. Insulin secretion is calculated as percent of total insulin and presented as percent induction by 100 nM Liraglutide, n = 3. D) qPCR measurement of GLP1R mRNA from cells treated with a shRNA control or a shRNA against the GLP1R; data normalized to TBP, n = 3. E) Dose response of GRP in concentrations 1 pM, 100 pM and 10 nM; n = 3. F) Dose response of NMB C in concentrations 1 pM, 100 pM and 10 nM; n = 3. G) Dose response of NMB B32 in concentrations 0.1 nM, 1 nM and 10 nM; n = 3. H) Dose response of NMB B in concentrations 0.1 nM, 1 nM and 10 nM; n = 3. I) Dose response of PACAP 27 in concentrations 1 nM, 10 nM and 100 nM, n = 3. J) Dose response of PACAP 38 in concentrations 1 nM, 10 nM and 100 nM, n = 3. Data are shown as mean + SEM, P-values are determined by unpaired One Way ANOVA and Student's t-test. The # symbol illustrates significant difference from low glucose; #∼p < 0.05; ##∼p < 0.01. The $ symbol illustrates significant difference from low glucose + Ex-4; $∼p < 0.05; $$∼p < 0.01. The * symbol illustrates significant difference from high glucose, *∼p < 0.05; **∼p < 0.01. The ° symbol illustrates significant difference from high glucose + Ex-4, °∼p < 0.05; °°∼p < 0.01.