Supplementary Figure S4.

Stimulation of EndoC-βH1 cell proliferation by the small molecule CHIR99021 and the proteins SerpinA6, STC1 and APOH. A) An image of the cells after EdU incorporation assay, EdU-positive cells (green) and DAPI (blue). B) Stimulatory effect of CHIR99021 after 24h treatment measured by EdU incorporation, n = 2–3; at least 10K cells were analyzed for each treatment. Dose response effect of SerpinA6, n = 3 (C); STC1, n = 2 (D) and APOH, n = 2 (E), measured by 14C-Thymidine incorporation after 48h treatment. Data are shown as mean + SEM. P-values determined by unpaired One Way ANOVA and Student's t-test. The * symbol illustrates significant difference from non-treated control cells, *∼p < 0.05; **∼p < 0.01.