SST expression in EndoC-βH1 cells and EndoC-βH1 pseudoislets. A) Immunocytochemical staining for SST (red), insulin (green) and DAPI (blue) in EndoC-βH1 cells, n = 3. B) SST ELISA in EndoC-βH1 cells, n = 3. C) Effect of 100 nM SST, 100 nM SST-receptor 2 (SSTR2) antagonist Cyn154806, and 1 μM SSTR5-antagonist BIM23056 in addition to high glucose on insulin secretion in EndoC-βH1 cells, n = 3–8. Data are shown as mean + SEM, P-values are determined by unpaired One Way ANOVA and Student's t-test. The # symbol illustrates significant difference from low glucose; #∼p < 0.05; ##∼p < 0.01. The * symbol illustrates significant difference from high glucose, *∼p < 0.05; **∼p < 0.01.