Table 1.
Summary table on SI and insulin secretion (presented as % of total insulin content) in EndoC-βH1 pseudoislets and monolayer cells during stimulation with low or high glucose with and without addition of Ex4. SI in EndoC-βH1 pseudoislets is calculated as AUC/min for a 38 min stimulation by perifusion. The presented values are averages from 3 to 9 independent experiments with 3–4 biological replicates per experiment. Values for amount of secreted insulin in human islets are obtained during stimulation with low glucose (range 1.8–3.5 mM) and high glucose (range 11.2–27 mM).
| EndoC-βH1/human islets | Low glucose |
Low glucose + Ex-4 |
High glucose |
High glucose + Ex-4 |
||||
|---|---|---|---|---|---|---|---|---|
| SI | Secreted insulin (%) | SI | Secreted insulin (%) | SI | Secreted insulin (%) | SI | Secreted insulin (%) | |
| EndoC-βH1 pseudoislets Perifusion |
– | 1.5 ± 0.1 | 1.5 ± 0.3 | 2.5 ± 0.4 | 4.4 ± 0.5 | 5.3 ± 0.7 | 9.0 ± 1.7 | 11.9 ± 2.6 |
| EndoC-βH1 pseudoislets Static GSIS |
– | 0.9 ± 0.3 | 2.4 ± 0.9 | 2.0 ± 0.7 | 8.5 ± 3.1 | 6.7 ± 0.1 | 14.2 ± 4.7 | 11.3 ± 1.0 |
| EndoC-βH1 monolayer Static GSIS |
– | 4.0 ± 2.0 | 2.5 ± 0.8 | 8.0 ± 4.4 | 2.0 ± 1.1 | 6.6 ± 1.6 | 3.3 ± 2.4 | 10.4 ± 4.2 |
| Human islets (6 studies) | – | 0.9–2.7 | – | – | – | 4.3–8.9 | – | – |