Figure 3.

Effect of ASC CM on the migration and invasion potential of T24 cells. (a) ASC CM harvested from a normal weight patient, an overweight patient, and an obese bladder cancer patient was placed in the bottom of a Transwell invasion chamber, and T24 cells were placed in the upper chamber. Migrated cells were stained and counted. ^∗ p < 0.05, ^∗∗ p < 0.01, and  ^∗∗∗ p < 0.001 are statistically significant. (b) ASC CM harvested from a normal weight patient, an overweight patient, and an obese bladder cancer patient was placed in the bottom of a Transwell Matrigel invasion chamber, and T24 cells were placed in the upper chamber. Invasive cells were stained and counted. ^∗ p < 0.05 is statistically significant.