Figure 3.

Regulatory T-cell (Treg) therapy in ocular disease: the original source of Tregs is the patient's peripheral blood. Following isolation of peripheral blood mononuclear cells (PBMCs) from the blood, PBMCs are cultured on anti-CD3-coated plates with RPE supernatants for 24 h. RPE supernatants are collected from culture media of human RPE cell lines with transforming growth factor beta 2 (TGF-β2) and high-dose interleukin 2 (IL-2). CD4⁺CD25⁺ T cells are first selected from cultured PBMCs. Sorted CD4⁺CD25⁺ T cells are then recultured with RPE supernatants together with recombinant IL-2 (rIL-2) and anti-CD3/CD28 antibodies for 72 h. In the final sort, CD4⁺CD25^highCD45RA⁻ T cells are collected, which are best suited for intravitreal injection into uveitis patients.