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. 2018 Spring;17(2):480–494.

Table 1.

Characteristics of the Primers Used in the Real-Time Polymerase Chain Reaction

Gene Sequence Amplicon Size (bp) Tm °C
GAPDH (F) ACTAACCCTGCGCTCCTG 87 59.03
GAPDH (R) CCCAATACGACCAAATCAGA 59.04
Bcl-XL (F) AACTGTGGTTCAGTGTGGGA 87 59.13
Bcl-XL (R) CTAGCTTCCTCCAAGATGGC 59.54
BCL-2 (F) CTTTACGTGGCCTGTTTCAA 88 58.68
BCL-2 (R) CTGAAGGACAGCCATGAGAA 58.90
BAX(F) AGCTGCAGAGGATGATTGC 153 59.43
BAX(R) GTTGAAGTTGCCGTCAGAAA 58.82