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Seed germination and callus induction of O. bolbutrichum. (A) germination of seeds on free growth factor MS medium (B) appearance of callus without pigment production on MS medium with 2.10 mg.L-1 kn and 1 mg.L-1 2, 4-D. (C) pigmented and non-pigmented calli on MS medium supplement with 2.10 mg.L-1 kn and 0.2 mg.L-1 2, 4-D. (D) dye formation in liquid 1/2MS medium containing 0.2 mg.L-1 IAA
