Table 2.
Phytochemical characteristics for various extracts of three different species of Scrophularia
| Phytochemical tests |
n-hexane extract
|
DCM extract
|
MeOH extract
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| S. f | S. s | S. a | S. f | S. s | S. a | S. f | S. s | S. a | ||
| Alkaloids | Dragendorff’s Test | - | - | - | - | - | - | + | - | - |
| Hager’s Test | - | - | - | - | - | - | + | - | - | |
| Cardiac Glycosides | Kedd | +++ | +++ | +++ | +++ | +++ | +++ | - | - | - |
| Keller-killiani | - | + | + | - | +++ | - | - | - | ||
| Tannins | Ferric Chloride Test | - | - | - | - | - | - | +++ | +++ | +++ |
| Flavonoids | Shinoda Test | - | - | - | ++ | - | - | +++ | +++ | +++ |
| Proteins and Amino Acids |
Ninhydrin Test | - | - | - | - | - | - | - | - | - |
| Sterol | Libermann-Buchard test | +++ | + | + | - | +++ | + | - | - | - |
| Terpenoid | Libermann-Buchard test | - | - | - | +++ | ++ | + | - | - | - |
| Carbohydrate | Benedict's test | - | - | - | - | - | - | + | + | + |
| Iridoids | Trim-Hill | - | - | - | - | - | - | + | + | + |
S. f = S. frigida; S. s = S. subaphylla; S. a = S. atropatana; MeOH = methanol; DCM = dichloromethane.