Figure 6.

Flow cytometry analysis of platelet activation. Expression levels of (A) P-selectin and (B) PAC-1 receptor (integrin α_IIbβ₃) in perfused blood samples were compared with unstimulated (negative control) and thrombin stimulated (positive control) samples collected prior to perfusion. Analysis of 100,000 events for each sample was conducted, and events of platelets expressing each marker were recorded. Statistical significance was obtained using paired t-test (n = 3, *p < 0.05 and **p < 0.005 relative to “no stimulation” control).