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. 2018 Jan 31;10:55–65. doi: 10.1016/j.molmet.2018.01.019

Figure 3.

Figure 3

Metabolic and mechanical adipose tissue in Ad-B2(−/−)mice. (A) Whole mouse tibiae stained with osmium tetroxide and (B) quantification of cMAT, rMAT, and total MAT by μCT in 16-week-old Ad-B2(+/−) and Ad-B2(−/−) mice (n = 4–5). Representative H&E staining of MAT from caudal vertebrae from the tail (C) and quantification of adipocyte area and frequency of caudal vertebrae MAT (D) of mice described in A. (E) Representative H&E staining images of IFP sections in 12-week-old Ad-B2(+/−) and Ad-B2(−/−) mice. Scale bar represents 100 μm. Quantification of IFP size (F) and adipocytes per section (G) of mice described in E (n = 7). (H) Bscl2 mRNA levels of 12-week-old Ad-B2(+/−) and Ad-B2(−/−) mice in IFP (n = 7). (I) Representative H&E staining images of IFP sections in WT and SKO mice. Scale bar represents 100 μm. All data are presented as the mean ± SEM, *p < 0.05.