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. 2018 Jan 31;10:55–65. doi: 10.1016/j.molmet.2018.01.019

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Physiological and metabolic phenotype of Ad-B2^(−/−)mice. (A) Energy expenditure (EE) and (B) respiratory exchange ratio (RER) values in Ad-B2^(+/−) and Ad-B2^(−/−) mice at 14 weeks of age fed a standard chow diet (n = 4–5). (C) Mice as described in B were challenged with a 9-h fast and then given ad libitum access to chow diet and RER values were collected throughout. Average RER values obtained during fasting (D) and re-feeding (E) periods (n = 4–5). Analysis of serum triglyceride (F), insulin (G), glucose (H), quantitative insulin sensitivity check index (QUICKI) analysis (I) and liver triglyceride levels (J) normalised to tissue weight in Ad-B2^(+/−) and Ad-B2^(−/−) mice fasted for 5 h at 16 weeks of age (n = 4–5). (K) Glucose tolerance test in Ad-B2^(+/−) and Ad-B2^(−/−) mice at 10 weeks of age (n = 4). Relative gene expression of white (L), brite (M) and brown (N) adipocyte markers in EWAT of Ad-B2^(+/−) and Ad-B2^(−/−) mice at 16 weeks of age (n = 4–5). All data are presented as the mean ± SEM, *p < 0.05.