Figure 3.

Leucine-induced changes in [Ca²⁺]_iare independent of mTORC1 signaling. Immunostaining against ribosomal protein S6 phosphorylated at Ser240/244 (p-rpS6) in primary cultures of mediobasal hypothalamic neurons treated for 1 h with 10 nM rapamycin (A). Neuronal responses to leucine in primary cultures of mediobasal hypothalamic neurons pre-treated with rapamycin (B). Leucine-induced changes in [Ca²⁺]_i as assessed by 340/380 nm fluorescence ratios in primary cultures of mediobasal hypothalamic neurons treated with rapamycin (C, D). Data are means ± SEM.