Figure 4.

Leucine-induced changes in [Ca²⁺]_iare independent of leucine metabolism and K_ATPchannels. Effect of KIC exposure on neuronal activation and inhibition (A). Neuronal responses to tolbutamide and leucine exposures in neuronal mediobasal hypothalamic cultures (B) or arcuate POMC neurons (C). Changes in [Ca²⁺]_i, as assessed by 340/380 nm fluorescence ratios in primary cultures of mediobasal hypothalamic neurons treated with leucine in the presence of Tolbutamide (D, E, F). Overlap between leucine-activated neurons and neurons sensitive to Tolbutamide (G). Effect of diazoxide (diaz) on leucine-induced neuronal activation and inhibition (H), and changes in [Ca²⁺]_i, as assessed by 340/380 nm fluorescence ratios in primary cultures of mediobasal hypothalamic neurons treated with leucine in the presence of diazoxide (I). Data are means ± SEM. *: p < 0.05. **: p < 0.01.