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. 2018 Feb 7;10:14–27. doi: 10.1016/j.molmet.2018.01.021

Figure 7.

Figure 7

Leucine sensing in hypothalamic neurons derived from human pluripotent stem cells. Immunofluorescent detection of POMC expression in hypothalamic neurons derived from human pluripotent stem cells (A). 340/380 nm fluorescence (B), peak 340/380 ratio (C) and 340/380 ratio AUC (D) in human hypothalamic neurons activated in response to 500 μM leucine exposure. 340/380 nm fluorescence (E), minimum 340/380 ratio (F) and 340/380 ratio AUC (G) in human hypothalamic neurons inhibited in response to 500 μM leucine exposure. Data are mean ± SEM. *: p < 0.05. **: p < 0.01. ***: p < 0.001.