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Linearity of variant allele fractions across serial dilutions. Serial dilutions were made of a cell line DNA mixture with 6 characterized mutations into negative control DNA. Four single nucleotide variants (SNVs) (BRAF p.V600E, KRAS p.G12V, JAK2 p.V617F, and TP53 p.R273H), 1 insertion (FLT3 p.D600_L601ins10) and 1 deletion (EGFR p.E746_A750del) were assessed. VAF, variant allele fractions.
