Table 4. Optimization of read length vs. indel call accuracy by in silico trimming.
Gene_bp_indel | Read length | |||
---|---|---|---|---|
300_bp | 250_bp | 225_bp | 200_bp | |
FLT3_30_ins | 0.16 | 0.17 | 0.17 | 0.15 |
FLT3_30_ins | 0.13 | 0.15 | 0.15 | 0.12 |
FLT3_36_ins | 0.36 | 0.37 | 0.37 | 0.30 |
FLT3_54_ins | 0.63 | 0.68 | 0.64 | 0.59 |
FLT3_78_ins | 0.01 | 0.01 | 0.02 | 0.01 |
FLT3_93_ins | 0.02 | 0.02 | 0.02 | Not detected |
FLT3_93_ins | 0.05 | 0.07 | 0.10 | Not detected |
KIT_6_delins | 0.31 | 0.40 | 0.40 | 0.40 |
NPM_4_ins | 0.45 | 0.45 | 0.45 | 0.45 |
NPM_4_ins | 0.46 | 0.46 | 0.46 | 0.46 |
NPM_4_ins | 0.44 | 0.45 | 0.45 | 0.45 |
NPM_4_ins | 0.4 | 0.40 | 0.40 | 0.40 |
NRAS_30_ins | 0.43 | 0.34 | 0.34 | 0.34 |