Table 5. Limit of detection for integrated microfluidics circuit (IFC) library preparation method.

IFC	BRAF_V600E			KRAS_G12V			EGFR E746_A750del			FLT3 D600_L601ins10			JAK2_V617F			TP53_R273H
	Predicted	Observed		Predicted	Observed		Predicted	Observed		Predicted	Observed		Predicted	Observed		Predicted	Observed
Straight	–	0.150		–	0.571		–	0.757		–	0.115		–	0.533		–	0.372
1:4	0.037	0.026		0.143	0.269		0.189	0.549		0.029	0.041		0.133	0.217		0.093	0.140
1:16	0.009	0.009		0.036	0.085		0.047	0.327		0.007	0.004		0.033	0.095		0.023	0.048
1:64	0.002	0.010		0.009	0.020		0.012	0.093		0.002	0.004		0.008	0.022		0.006	0.021