Table S2. Accuracy of VAF results with downsampling by amplicon to maximum 7,500× coverage.

Sample type	Variant (VAF) without downsampling	VAF downsampling cutoff =7,500
Fixed	BRAF V600R (0.86; 2 bp indel)	0.87
Unfixed	CALR L367Tfs*46 (0.44)	0.47
Fixed	KRAS G12G (0.22)	0.24
Unfixed	FLT3 ITD (0.05; 90 bp ins); NPM1 Y288Cfs (0.39)	0.06; 0.40
Fixed	EGFR p.L747_T751del (0.39)	0.37
Fixed	KIT Q556_K558delinsH (0.29)	0.30
Fixed	NRAS Q61L (0.41)	0.37
Unfixed	BRAF V600E (0.14); EGFR E746_A750del (0.75); KRAS G12V (0.38)	0.17; 0.75; 0.38