Table S3. Intra-run replicate precision.
Sample_1 | Sample_2 | Concordant variants | Discrepant variants | Cause(s) of discrepancy |
---|---|---|---|---|
Integrated microfluidics circuit (IFC) | ||||
BM1_DUP | BM1 | 8 | 0 | |
PB1_DUP | PB1 | 8 | 0 | |
CELL_DUP | CELL | 16 | 3 | Intronic homopolymer [1]; single nucleotide variants (SNV) threshold [1]; indel mapping [1] |
FFPE1_DUP | FFPE1 | 6 | 5 | Intronic homopolymer [5] |
FFPE2_DUP | FFPE2 | 6 | 1 | Intronic homopolymer [1] |
FFPE3_DUP | FFPE3 | 7 | 6 | Intronic homopolymer [6] |
FFPE4_DUP | FFPE4 | 6 | 5 | Intronic homopolymer [5] |
Low input | ||||
FFPE9_DUP | FFPE9 | 1 | 0 | |
FFPE10_DUP | FFPE10 | 1 | 0 | |
FFPE11_DUP | FFPE11 | 0 | 0 | |
FFPE12_DUP | FFPE12 | 0 | 0 | |
FFPE13_DUP | FFPE13 | 0 | 0 | |
FFPE14_DUP | FFPE14 | 1 | 0 | |
FNA1_DUP | FNA1 | 1 | 0 | |
FNA2_DUP | FNA2 | 1 | 0 | |
FNA3_DUP | FNA3 | 1 | 0 | |
FNA4_DUP | FNA4 | 0 | 0 | |
FNA5_DUP | FNA5 | 0 | 0 | |
FNA6_DUP | FNA6 | 0 | 0 |