Figure 4.

Complexome Profiling

Heatmap representation (top) of the migration profiles of mt-SSU and mt-LSU in control, S1, and S3 fibroblasts. Interaction heatmaps were created by hierarchical clustering, including the manual addition of known ribosomal components that were not grouped together by the algorithm. Top-center and top-right panels show decreased amounts of fully assembled mt-SSU in both S1 and S3 fibroblasts and a subassembly of eight subunits present in control and S1 cells but absent in S3 cells. The abundance of mt-LSU is unaffected in S1 and S3. Migration profiles (bottom) of both mitoribosomal subunits show the relative abundance of proteins plotted against their apparent molecular mass and reveal decreased abundance of mt-SSU and unaffected abundance of mt-LSU in both subjects. The relative abundance was calculated as the average of the normalized iBAQ values for all mt-SSU or mt-LSU proteins.