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. 2018 May 28;9:1164. doi: 10.3389/fimmu.2018.01164

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Copyright © 2018 Kidane, Mitra, Wernsdorf, Hess and Liebhart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virulent and attenuated histomonads localized in cecum and liver by in situ hybridization. Stained histomonads could be seen in dark purple (examples shown in red arrow). Virulent histomonads could be seen in larger number, whereas only a few attenuated histomonads could be detected surrounded by an influx of mononuclear cells. Samples are representatives for tissues collected from turkeys inoculated with virulent histomonads (group infected turkey) necropsied on 10 days post-inoculation (DPI) and turkeys inoculated with only attenuated histomonads (group vaccinated turkeys) necropsied on 21 DPI. Insets show higher magnifications of histomonads and the surrounding cells in the respective tissue sample.