Skip to main content
. 2018 May 28;11:177. doi: 10.3389/fnmol.2018.00177

FIGURE 5.

FIGURE 5

EEN1 is required for the structural and functional plasticity of dendritic spines. (A) Cultured EEN1+/+ and EEN1-/- hippocampal neurons were transfected with pLL3.7.1 on DIV12-13 to express DsRed as volume marker, fixed and immunostained for EEN1 and DsRed on DIV19. Shown are representative confocal images of dendrites. (B) Quantification of EEN1 fluorescent signals in spines in A, normalized to levels of EEN1+/+ neurons. Data represent mean ± SEM, n > 10 neurons, >600 spines per group, ∗∗∗p < 0.001. (C) Cultured EEN1+/+ and EEN1-/- neurons co-transfected with DsRed expression construct and Flag vector, and EEN1-/- neurons co-transfected with constructs expressing DsRed- and Flag-tagged EEN1, EEN2, or EEN3 on DIV12-13 were treated with glycine to induce chemLTP with or without MK801 pretreatment on DIV18, and immunostained for surface GluA1, Flag, and DsRed. Shown are representative confocal images of dendrites. (D) Quantification of spine density in C. (E) Changes of spine density in C. (F) Quantification of spine head area in C. (G) Changes of spine head area in C. (H) Quantification of surface GluA1 levels in spines in C. (I) Changes of surface GluA1 levels in spines in C. Data represent mean ± SEM in D–I, n > 15 neurons per group, >850 spines per group, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars, 2 μm.