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. 2018 May 28;9:547. doi: 10.3389/fphys.2018.00547

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Copyright © 2018 Pisciotta, Bertoni, Riccio, Mapelli, Bigiani, La Noce, Orciani, de Pol and Carnevale.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Immunofluorescence analysis on immunomagnetically selected hDPSCs shows the expression of the stemness markers c-Kit, STRO-1 and CD34. Bar: 10 μm. (B) Phase contrast images demonstrate the ability of selected hDPSCs to aggregate in floating three-dimensional spheres, as early as 24 h after cell seeding, and to grow in size through culture time, up to passage 8. On the right, a higher magnification emphasizing the semi-transparent appearance of 3D spheres. Bar: 100 μm.