Table 1.
Bacterial strains and plasmids.
| Strain | Description | |
|---|---|---|
| 2457T | Wild-type S. flexneri 2a | |
| CVD 1208S | ΔguaBA, Δsen, Δset S. flexneri 2a vaccine derived from 2457T | |
| J17B | Wild-type S. flexneri 3a | |
| CVD 1213 | ΔguaBA, Δsen S. flexneri 3a vaccine, derived from J17B | |
| CCH060 | Wild-type S. flexneri 6 | |
| CVD 1215 | ΔguaBA S. flexneri 6 vaccine derived from CCH060 | |
| 4243A | Plasmid cured S. flexneri 2a derived from 2457T | |
| Plasmid | Description | Reference |
| pFM726A.02 | Suicide plasmid containing kanamycin resistance cassette, guaA and guaB fragments | Noriega et al. (1996) |
| pKD46 | Plasmid encodes lambda red genes; temperature sensitive replication; ampicillin resistance | Datsenko and Wanner (2000) |
| pCP20 | FLP recombinase expression plasmid (temperature dependent), ampicillin and chloramphenicol resistance | Datsenko and Wanner (2000) |
| pKD3 | Plasmid used for gene disruption; flanks FRT sites; temperature sensitive; chloramphenicol resistance | Datsenko and Wanner (2000) |
| pKD4 | Temperature sensitive, kanamycin resistance | Datsenko and Wanner (2000) |