Figure 1.

Pilin gene expression as determined by RNA sequencing of C. difficile samples grown on blood agar under conditions that express pili (A) as planktonic culture (B), and as a biofilm on glass beads (C). Using a three-way comparison, pilin gene expression is shown as a heatmap (D), and differential expression between the three growth conditions is shown using pairwise comparisons (E). *q value < 0.05. Please note that data included in panel A were previously published (Piepenbrink et al.2015).