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. Author manuscript; available in PMC: 2018 Jun 4.
Published in final edited form as: Biochemistry. 2017 Feb 9;56(7):971–976. doi: 10.1021/acs.biochem.6b01251

Figure 1.

Figure 1

A34F GB1 dimer and monomer and 19F NMR spectra acquired at two concentrations. In each spectrum, the area under a peak is proportional to the concentration of the corresponding state, allowing straightforward quantification of the dissociation constant, KD→M.