Table 1.
KD→M and ΔGo′D→M values for the A34F GB1 Dimer at pH 7.5, 298 Ka
| condition |
μM
|
kcal/mol
|
|
|---|---|---|---|
| KD→M | ΔG°′D→M | ΔΔG°′D→M | |
| buffer (20 mM NaPO4) | 59 ± 2 | 5.75 ± 0.03 | N/A |
| ethylene glycol, 200 g/L | 95 ± 6 | 5.46 ± 0.04 | −0.29 ± 0.05 |
| urea, 100 g/L | 90 ± 5 | 5.50 ± 0.03 | −0.25 ± 0.04 |
| 8 kDa PEG, 200 g/L | 85 ± 5 | 5.53 ± 0.03 | −0.22 ± 0.03 |
| lysozyme, 50 g/L | 80 ± 5 | 5.57 ± 0.04 | −0.18 ± 0.05 |
| sucrose, 300 g/L | 55 ± 3 | 5.78 ± 0.03 | 0.03 ± 0.04 |
| Ficoll-70, 300 g/L | 45 ± 2 | 5.90 ± 0.03 | 0.15 ± 0.04 |
| BSA, 100 g/L | 26 ± 2 | 6.23 ± 0.05 | 0.48 ± 0.06 |
| TMAO, 38 g/L | 25 ± 2 | 6.25 ± 0.05 | 0.50 ± 0.06 |
| cytosol, 75 g/L | 17 ± 2 | 6.47 ± 0.07 | 0.72 ± 0.08 |
a
Values are averages. Uncertainties are standard deviations of the mean from three trials.