Table 1.

Free energies of unfolding $(\mathrm{\Delta }{G}_U^{{°}^{\prime }})$ at 298 K with standard deviations of the mean from three trials

Condition	$(\mathrm{\Delta }{G}_U^{{°}^{\prime }})$, (kcal/mol)
Buffer
D2O (pH 7.8)	1.5 ± 0.1
D2O + 0.3 M NaCl	1.7 ± 0.2
D2O + 0.3 M NaCl +1 mM glycine betaine	1.8 ± 0.1
H2O (pH 7.8)	0.63 ± 0.03
H2O (pH 7.8), 0.68 m glycine betaine	1.1 ± 0.1
Cells
D2O	1.6 ± 0.2
D2O + 0.3 M NaCl	0.54 ± 0.08
D2O + 0.3 M NaCl +1 mM glycine betaine	1.5 ± 0.2