Table 1.
Dissociation constants measured for binding of PKCα–C2 to vesicles containing PA derivatives.
| POPC:POPE:PA (40:40:20) | Kd (nM)a |
|---|---|
| Synthetic PA (POPA) | 440 ± 50 |
| Azido PA (1b) | 640 ± 140 |
| Naphthyl PA (12a) | 780 ± 50 |
| Dansyl PA (12b) | 800 ± 70 |
| Coumarin PA (12c) | 850 ± 120 |
| Rhodamine PA (12d) | 510 ± 90 |
| Benzophenone PA (12e) | 570 ± 150 |
| Benzophenone/Azido PA (12f) | 520 ± 80 |
| POPC:POPE:PA (55:40:5) | Kd (nM)a |
| Synthetic PA (POPA) | 490 ± 90 |
| Azido PA (1b) | 730 ± 130 |
| Coumarin PA (12c) | 940 ± 120 |
| Rhodamine PA (12d) | 810 ± 80 |
a
Binding experiments were performed in 25 mM Tris HCl pH 7.4, 150 mM NaCl, 1 mM CaCl2 and 1 mM DTT