Skip to main content
. 2018 Feb 1;102(2):278–295. doi: 10.1016/j.ajhg.2018.01.006

Figure 2.

Figure 2

Df(h15q13)/+ Mice Have Defects in Dendritic Spine Development and Neuronal Morphology

(A) Golgi-stained dendritic spine images (left) and spine density analysis (right). Df(h15q13)/+ mice show a decrease in spine density in layer II/III PFC neurons. WT, n = 40 dendritic segments, 22 neurons; Df(h15q13)/+, n = 40 dendritic segments, 26 neurons, 4 mice per condition, Student’s t test, ∗∗p < 0.01, t(78) = 2.846. Scale bar = 5 μm.

(B) Df(h15q13)/+ mice show a decrease in PFC mushroom spines (left). WT, n = 40 dendritic segments, 22 neurons; Df(h15q13)/+, n = 40 dendritic segments, 26 neurons, 4 mice per condition, Student’s t test, ∗p < 0.05, t(78) = 2.403, t(78) = 0.1312, t(78) = 1.673.

(C) Traces of P28 WT and Df(h15q13)/+ Golgi-stained layer II/III prefrontal cortical (PFC) neurons.

(D) Sholl analysis (left) and the total number of intersections (right). Df(h15q13)/+ mice show a decrease in dendrite growth in layer II/III PFC neurons. n = 40 neurons, 4 mice per condition, two-way ANOVA followed by Tukey’s post hoc test, ∗∗∗p < 0.001, F(9, 780) = 109.2, t(78) = 3.449.

(E) Dendritic spine images from WT and Df(h15q13)/+ cultured neurons (scale bar = 5 μm).

(F) Spine density measurements in cultured neurons. Spine density is decreased in Df(h15q13)/+ cultured cortical neurons. WT, n = 32 dendritic segments from 12 neurons; Df(h15q13)/+, n = 32 dendritic segments from 15 neurons, 3 cultures, Student’s t test, ∗p < 0.05, t(62) = 2.311.

(G) Dendritic spine classification in WT and Df(h15q13)/+ neurons. In Df(h15q13)/+ cultured cortical neurons, the proportion of mushroom-type spines is decreased (left) and the proportion of stubby type spines is increased (right). WT, n = 32 dendritic segments from 12 neurons; Df(h15q13)/+, n = 32 dendritic segments from 15 neurons, 3 cultures, Student’s t test, ∗p < 0.05, ∗∗p < 0.01, t(62) = 2.007, t(62) = 0.1383, t(62) = 2.875.

(H) DIV14 WT and Df(h15q13)/+ cultured cortical neurons expressing Venus (scale bar = 50 μm).

(I) Sholl analysis of cultured cortical neurons. Dendrite growth is decreased in Df(h15q13)/+ neurons. n = 50 neurons, 3 cultures, two-way ANOVA followed by Sidak post hoc test, ∗∗∗p < 0.001, F(14, 1470) = 85.55. Error bars represent SEM.