Figure 5.

TBS^{p.Pro332Hisfs∗10} Cells Show Changes in the Localization of CCP110 and CEP97

(A and C) Immunofluorescence micrographs of cycling human-derived fibroblasts stained with antibodies against endogenous CCP110 (A) or CEP97 (C) (green), CEP164 to label the mother centriole (MC; purple), and DAPI to label the nuclei (blue). Black and white images show the single green and purple channels. Note the different distribution of CCP110 and CEP97 to the MC between TBS^{p.Pro332Hisfs∗10} fibroblasts (TBS³³²) and control HFFs.

(B and D) Graphical representation of the percentage of cells showing the presence of CCP110 or CEP97 at the MC per micrograph corresponding to the experiments in (A) or (C), respectively; n = 30 micrographs. Three independent experiments were pooled together. Pictures were taken with a Zeiss Axioimager D1 fluorescence microscope with a 63× objective. All graphs represent the mean and SEM. Scale bars, 1 μm.