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. 2018 Jun 1;32:2058738418772652. doi: 10.1177/2058738418772652

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — MiR-21-5p directly targeted BISPR. (a) TargetScan predicted that there was a binding site of miR-21-5p existing in the 3′-UTR of BISPR. (b) Relative luciferase activity in BISPR-wt and miR-21-5p mimics group was lower than that in BISPR-wt and miR-NC group. There was no significant difference between BISPR-mut and miR-21-5p mimics group and BISPR-mut and miR-NC group. (c) MiR-21-5p expression in BHT101 and Hth83 cell lines with Bio-BISPR-probe was notably higher than that in Bio-NC probe group. (d) MiR-21-5p expression in tumor tissues was lower than that in adjacent tissues. (e) Compared with sh-NC group, miR-21-5p expression in BHT101 and Hth83 cell lines with sh-BISPR was much higher than that in sh-NC group. ^*P < 0.05, compared with sh-NC group.