Table II.
Selected examples of Arabidopsis and oilseed rape expression patterns
| Putative Identitya | Clone ID | Ratiob
|
||||
|---|---|---|---|---|---|---|
| Seed/leaf
|
Seed/root
|
|||||
| B.n.c | A.t.1d | A.t.2 | A.t.1 | A.t.2 | ||
| Storage proteins | ||||||
| 12S Storage protein | M29F06 | 25 | 66 | 92 | 27 | 21 |
| 2S Storage protein 4 | M15F11 | 16 | 28 | 62 | 13 | 18 |
| Lipid metabolism | ||||||
| Biotin carboxyl carrier protein (oilseed rape) | M53F09 | 2 | 5 | 8 | 4 | 4 |
| ω-3 Fatty acid desaturase (endoplasmic reticulum) | M43E07 | 7 | 4 | 3 | 4 | 3 |
| Fatty acid elongase (FAE1) | M16H06 | 16 | 6 | 8 | 8 | 7 |
| Diacylglycerol acyltransferase | E6B2T7 | 2 | −2 | 3 | 2 | −2 |
| Lipoxygenase (Solanum tuberosum) | M30E03 | 6 | 14 | 19 | 18 | 12 |
| Lipid transfer protein 2 | M44A11 | 2 | 11 | 11 | 8 | 10 |
| Oleosin | M36H07 | 41 | 55 | 59 | 35 | 32 |
| Carbohydrate metabolism | ||||||
| Pyruvate dehydrogenase E1 alpha subunit | M11E10 | 13 | 8 | 12 | 8 | 10 |
| Transcriptional regulation | ||||||
| Pc-Myb2-like protein | M18D02 | 3 | 11 | 25 | 16 | 15 |
| Zinc finger protein | M18H11 | 7 | 17 | 23 | 18 | 12 |
| CCAAT-box binding factor HAP3 | M38G03 | 2 | 4 | 6 | 6 | 5 |
| Signal transduction | ||||||
| grr1 Homolog (Glycine max) | M41C07 | 12 | 15 | 16 | 14 | 13 |
| Calmodulin-related protein 2 | M12B04 | 8 | 42 | 50 | 24 | 21 |
| Receptor protein kinase homolog | M19D10 | 12 | 19 | 38 | 31 | 14 |
| Putative protein Ser/Thr kinase | M34C01 | 3 | 12 | 14 | 10 | 8 |
| Putative ARF1 GTPase-activating protein | M39G12 | 3 | 2 | 2 | 16 | 9 |
| Development | ||||||
| Endosperm-specific protein (Zea mays) | M60F09 | 1 | 4 | 2 | 3 | 3 |
| Embryo-specific protein 1 | M58H11 | 24 | 13 | 23 | 28 | 16 |
| Embryo-specific protein 3 | M49A09 | 3 | 28 | 24 | 22 | 16 |
| Terminal flower 1 | M36F04 | 5 | 17 | 37 | 16 | 17 |
| Dessication-related protein (Craterostigma plantagineum) | M12D06 | 3 | 22 | 37 | 28 | 12 |
| Photosynthesis | ||||||
| RuBisCo SSU | M54F03 | −18 | −8 | −7 | 11 | 18 |
| Chla/b-binding protein | M18A12 | −5 | −5 | −4 | 17 | 12 |
Identity from BLAST analysis. Organisms other than Arabidopsis are given behind the putative gene identities.
Fluorescence intensity ratios of seed versus leaf and seed versus root experiments. Repeated experiments contained the Cy3/Cy5 fluorescence dyes in opposite orientation. The highest ratios observed for fluorescence intensity of seed versus leaf or root probes were 50 to 100. These ratios likely underestimate the true expression ratio for genes such as seed storage proteins and thus reflects the nature of microarray data and the methods used to deal with background fluorescence.
B.n., Experiment with probe from oilseed rape.
A.t., Experiments with probes synthesized from Arabidopsis tissues.