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Comparison of fluorescent (Left) and qPCR (Right) methods for analyzing dsRNA degrading nucleases in S. litura gut fluid. Serial dilutions of the gut fluid (20, 40, 80, 160, 320, and 640 times) were incubated with 1 μL 24 bp fluorescence labeled dsEGFP or 1 μL 414 bp naked dsEGFP in a total volume of 20 μL at 37°C. The final substrate concentration was 0.5 μM for fluorescence method and 0.05 μg/μL for qPCR method. Values are mean ± SE; n = 3.
