FIGURE 6.

Distinct impacts of Mg²⁺ concentrations on the dsRNA degrading nucleases of four insects. The homogenates were prepared from whole body of different insects. Tested conditions were conducted under their optimal pH (pH 10.0 for S. litura and pH 9.0 for the other three species). Each reaction containing 19 μL enzyme solution and 1 μL fluorescence labeled dsRNA substrate at a final concentration of 0.5 μM. The fluorescence intensity in different reactions were continuously monitored at 37°C. Values are mean ± SE; n = 3.