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DNA amplification curves with different concentration of the inner primer in DS-LAMP (A) and Bühlmann-LAMP (B). Concentrations of outer primers and loop primers were fixed at 0.2 μM and 0.8 μM, respectively. The genomic DNA of Ea YKB12316 was used as a DNA template, and LAMP assays were carried out at 65°C for 30 min.
