Fig. 3.

DS-LAMP detection sensitivity to Erwinia amylovora from the sample extracts. The 0.5 ml of serially diluted suspensions of E. amylovora YKB 12316 (10⁸–10⁴ cells/ml) was pitched in 4.5 ml antioxidant maceration buffer containing subcortical tissues (0.1–0.2 g) of a healthy pear twig placed in a plastic bag. Outside of the plastic bag was hammered lightly with the lubber hammer, and 1 μl of the extract was used for DS LAMP (A). Regression curve (B) was y = −2.285x + 23.325, and R² was 0.9836.