Table 3.
On-site diagnosis of fire blight with DS-LAMP and Bühlmann-LAMP assays
| Location | Host | Samplea | LAMPb | Isolationc | |
|---|---|---|---|---|---|
|
| |||||
| Bühlmann | DS | ||||
| Anseong, Seoun 1 Dec 13, 2016 |
pear | Twig with canker-1 | + | + | Ea |
| Twig with canker-2 | + | + | Ea | ||
| Twig with canker-3 | + | + | Ea | ||
| Healthy twig | + | − | − | ||
| Sejong, Bugang Dec 21, 2016 |
pear | Twig with canker-1 | − | − | − |
| Twig with canker-2 | − | − | − | ||
| Twig with canker-3 | − | − | − | ||
| Branch with canker-4 | − | − | − | ||
| Branch with canker-5 | − | − | − | ||
| Anseong, Seoun 2 Jan 11, 2017 |
pear | Twig with canker -1 | + | + | Ea |
| Twig with canker -2 | + | + | Ea | ||
| Healthy twig -1 | − | − | − | ||
| Healthy twig -2 | − | − | − | ||
| Anseong, Miyang May 17, 2017 |
pear | Blacken Leafstalk | + | + | Ea |
| Twig with canker | + | + | Ea | ||
| Trunk with canker | + | + | Ea | ||
| Healthy leafstalk | + | − | − | ||
| Healthy twig | − | − | − | ||
| Healthy leafstalk | − | − | − | ||
| Healthy branch | − | − | − | ||
| Cheonan, Seobuk Jun 8, 2017 |
apple | Ooze on fruitlet | + | + | Ea |
| Twig with canker | + | + | Ea | ||
| Trunk with canker | + | + | Ea | ||
| Ooze on twig | + | + | Ea | ||
| Blacken leafstalk | + | + | Ea | ||
| Healthy twig | − | − | − | ||
| Kwangju, Namjong Jun 14, 2017 |
apple | Twig with canker | − | − | Ep |
| Blacken leafstalk | − | − | Ep | ||
| Twig with canker | − | − | Ep | ||
| Branch with canker | − | − | Ep | ||
| Healthy twig-1 | − | − | − | ||
| Healthy twig-2 | − | − | − | ||
a
Healthy plant samples collected from healthy trees near diseased trees with typical symptoms
b
+, DNA amplification within 30 min by LAMP assays; −, no DNA amplification within 30 min by LAMP assays
c
Isolated pathogens from the sample extracts. Ea, E. amylovora; Ep, E. pyrifoliae; −, no pathogen isolated